Production of Staphylococcal Alpha Toxin II. Glucose Repression of Toxin Formation
نویسندگان
چکیده
منابع مشابه
Ultracentrifugal analysis of staphylococcal alpha toxin.
Ultracentrifugal examination of staphylococcal alpha toxin at different stages of purification showed the presence of a major component having a sedimentation coefficient of 2.8S, present to the extent of more than 90% of the sample, and identifiable with active toxin. Several minor components having S(20,w) values of 11.5S, 8.5S, and 2.0S were detected. The 11.5S component presumably is identi...
متن کاملThe H35A mutated alpha-toxin interferes with cytotoxicity of staphylococcal alpha-toxin.
Staphylococcal alpha-toxin is an important virulence factor for Staphylococcus aureus to cause severe infections. In this study, we explored whether the toxoid of alpha-toxin may be utilized to block the toxicity of wild-type alpha-toxin. We created a series of H35A mutated alpha-toxin expression strains and revealed that the H35A mutation eliminates the activity of alpha-toxin using a human lu...
متن کاملProduction of alpha toxin of Clostridium perfringens. II. Failure to produce a potent alpha toxin in the simplified media.
production of alpha toxin. Various kinds of enzymatic digests of protein have been used as a source of •gtoxin toxin factor•h (Adams et al., 1945; Logan et al., 1945; van Heyningen, 1948). Adams, Hendee and Pappenheimer (1947) showed that at least two factors present in the enzymatic digests of certain proteins and extracts of pancreas were necessary for the maximum production of alpha toxin in...
متن کاملPurification of staphylococcal alpha-toxin by adsorption chromatography on glass.
Staphylococcal alpha-toxin was purified from Staphylococcus aureus growth medium using adsorption chromatography on controlled pore glass beads. Elution of alpha-toxin from the unmodified glass surface of the beads with various anions generally followed the chaotropic series. Alpha-toxin, purified by glass bead chromatography, is composed of a single electrophoretic form, containing less than 2...
متن کاملInteraction of staphylococcal alpha toxin and the estrogenic hormone diethylstilbestrol.
Previous work in this laboratory showed that diethylstilbestrol was capable of suppressing induced furunculosis in rabbits. The present study indicates that the synthetic estrogenic hormone diethylstilbestrol which is used for acne, estrogen deficiency, cancer, and other disorders, can reduce the cytolytic action of staphylococcal alpha toxin. The cytotoxic action of purified alpha toxin for ti...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1972
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.6.5.689-694.1972